Miho INOUE-MURAYAMA (Professor)

Doctor of Science, Kyoto University

To learn more about the reproduction of animals in zoos and aquariums we are applying methods and analyses used to ascertain genetic diversity. Results of this work will not only benefit captive animals but can also be applied to conservation of species in the wild. To understand the range of genetic diversity within species requires obtaining and genotyping many samples for each species. We collected DNA samples from over 23,000 individuals representing 200 species of mammals. Since some animals are difficult to capture, and thus blood samples are not always available, we devised methods for efficient DNA analysis using noninvasive samples such as feces and hair. We stored these in a DNA database (the DNA Zoo), which also includes information on the geographical region in which the sample was collected and characteristics of the individual. We are going to develop The DNA Zoo so that it is able to link to cell bank. Using the DNA Zoo, we are identifying subspecies/populations/kinships by genotyping polymorphic markers such as microsatellites and mitochondrial DNA. Moreover, by analyzing genes on the sex chromosome, we can identify the sex of some bird species in which males and females are difficult to distinguish by observation alone. We are also surveying individual differences in functional genes related to personality traits (e.g. stress susceptibility) and reproduction. Introduction of the next-generation sequencer made marker isolation and meta-genome analysis easier, and widens the possibility of genome analysis of wild animals.

Positions held

  • 1987-1992 Primate research Institute, Kyoto University PhD course, Japan
  • 1990-1992 Researcher, Japan Society for the Promotion of Science, Japan
  • 1992-1994 Part-time lecturer, Primate research institute, Kyoto University, Japan
  • 1993-1997 Researcher, Shirakawa Institute of Animal Genetics, Japan LivestockTechnology Association, Japan
  • 1997-2003 Assistant Professor, Faculty of Agriculture, Gifu University, Japan
  • 2003-2004 Associate Professor, Faculty of Agriculture, Gifu University, Japan
  • 2004-2008 Associate Professor, Faculty of Applied Biological Sciences, Gifu University, Japan
  • 2008-present Professor, Wildlife Research Center, Kyoto University, Japan

Selected publications

  1. Ito H, Langenhorst T, Ogden R, Inoue-Murayama M: Population genetic diversity and hybrid detection in captive zebras. Scientific Reports, 5:13171, 2015.
  2. Weiss A, Staes N, Pereboom JJM, Inoue-Murayama M, Stevens JMG, Eens M: Personality in Bonobos. Psychological Science 26:1430]1439, 2015.
  3. Nishina K, Takagishi H, Inoue-Murayama M, Takahashi H, Yamagishi T: Polymorphism of the oxytocin receptor gene modulates behavioral and attitudinal trust among men but not women. PLoS One. 2015. 10:e0137089.
  4. Kaneko T, Ito H, Sakamoto H, Onuma m, Inoue-Murayama M: Sperm preservation by freeze-drying for the conservation of wild animals. PLOS ONE,e113381.2014
  5. Adenyo C, Hayano A, Inoue E, Kayang BB, Inoue-Murayama M. Development of microsatellite markers for grasscutter (Thryonomys swinderianus, RODENTIA) using next-generation sequencing technology. Conservation Genetics Resources, 4:1011-1014, 2012
  6. Fukuda T, Kurota J, Saito T, Yusasa K, Kurita M, Donai K, Nitto H, Soichi M, Nishimori K, Uchida T, Isogai E, Onuma M, Sone H, Seko N, Inoue-Murayama M: Efficient establishment of primary fibroblast cultures from the hawksbill sea turtle (Eretmochelys imbricata). In Vitro Cellular & Developmental Biology - Animal 48:660-665, 2012.
  7. Inoue-Murayama M, Weiss A, Morimura N, Tanaka M, Yamagiwa J, Idani G: Molecular Behavioral Research in Great Apes. In: Inoue-Murayama M, Kawamura S, Weiss A, editors. From Genes to Animal Behavior. Tokyo: Springer (ISBN978-4-431-53891-2) pp.239-253, 2011.
  8. Hong KW, Weiss A, Morimura N, Udono T, Hayasaka I, Humle T, Murayama Y, Ito S, Inoue-Murayama M: Polymorphism of the tryptophan hydroxylase 2 (TPH2) gene is associated with chimpanzee neuroticism. PLoS One. 6:e22144, 2011.
  9. Konno A, Inoue-Murayama M, Hasegawa T.: Androgen receptor gene polymorphisms are associated with aggression in Japanese Akita Inu. Biol Lett. 7:658-660, 2011
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